The MTT assay will be performed to determine the cell viability of cancer cells.
1. Seed approximately 10,000 cells/well will be seeded in 96-well plates.
2. Keep in the incubator at 37 ◦C under an atmosphere containing 5% CO2.
3. After overnight incubation to allow them to adhere, the cells will be fed with fresh medium containing varying concentrations of the respective compound and further incubated in different h.
4. Briefly, at the stipulated time following the treatment of the test compound, the medium will be aspirated and MTT (5 mg/mL) will be added in each well of the 96-well culture plate, and incubation will continue at 37 ◦C for 2-3 h.
5. The plates will be spun, supernatants will be discarded, and purple-colored formazan precipitates will be dissolved in 100 μL of dimethylsulfoxide (DMSO).
6. The color absorbance will be recorded at 590 nm on a microplate reader (BioTek Instruments, Inc. USA).
7. IC50 concentration (concentration at which the biological response is reduced by 50%) values will be determined for the cell viability inhibitory potential of the test compounds.
